Soybean variety S06-02KG294679

ABSTRACT

The present invention is in the field of soybean variety S06-02KG294679 breeding and development. The present invention particularly relates to the soybean variety S06-02KG294679 and its progeny, and methods of making S06-02KG294679.

REFERENCE TO RELATED APPLICATION

This is a continuation of prior application Ser. No. 11/362,025, filedFeb. 24, 2006, and now abandoned.

THE FIELD OF THE INVENTION

The present invention is in the field of soybean variety S06-02KG294679breeding and development. The present invention particularly relates tothe soybean variety S06-02KG294679 and its progeny, and methods ofmaking.

BACKGROUND OF THE INVENTION

Soybean Glycine max (L) is an important oil seed crop and a valuablefield crop. However, it began as a wild plant. This plant and a numberof other plants have been developed into valuable agricultural cropsthrough years of breeding and development. The pace of the developmentof soybeans, into an animal foodstuff and as an oil seed hasdramatically increased in the last one hundred years. Planned programsof soybean breeding have increased the growth, yield and environmentalhardiness of the soybean germplasm.

Due to the sexual reproduction traits of the soybean, the plant isbasically self-pollinating. A self-pollinating plant permits pollen fromone flower to be transferred to the same or another flower of the sameplant. Cross-pollination occurs when the flower is pollinated withpollen from a different plant; however, soybean cross-pollination is arare occurrence in nature.

Thus the growth and development of new soybean germplasm requiresintervention by the breeder into the pollination of the soybean. Thebreeders' methods of intervening in the pollination depend on the typeof trait that is being bred. Soybeans are developed for a number ofdifferent types of traits morphological (form and structure),phenotypical, or for traits like growth, day length, temperaturerequirements, initiation date of floral or reproductive development,fatty acid contents, insect resistance, nematode resistance, herbicideresistance and yield. The genetic complexity of the trait often drivesthe selection of the breeding method.

Due to the number of genes within each chromosome, millions of geneticcombinations exist in the breeders' experimental soybean material. Thisgenetic diversity is so vast that a breeder cannot produce the same twocultivars twice using the exact same starting parental material. Thus,developing a single variety of useful commercial soybean germplasm ishighly unpredictable, and requires intensive research and development.

The development of new soybeans comes through breeding techniques, suchas: recurrent selection, mass selections, backcrossing, single seeddescent and multiple seed procedure. Additionally, markers' assistedbreeding allows more accurate movement of desired alleles or evenspecific genes or sections of chromosomes to be moved within thegermplasm that the breeder is developing. RFLP, RAPD, AFLP, SSR, SNP,SCAR, isozymes, are all forms of markers that can be employed inbreeding soybeans or in moving traits into soybean germplasm. Otherbreeding methods are known and are described in various soybeantextbooks.

When a soybean variety is being employed to develop a new soybeanvariety or an improved variety the selection methods includebackcrossing, pedigree breeding, recurrent selection, modified selectionand mass selection. The efficiency of the breeding procedure along withthe goal of the breeding are the factors for determining which selectiontechniques are employed. A breeder continuously evaluates the success ofthe breeding program and therefore the efficiency of any breedingprocedure. The success is usually measured by yield increase, commercialappeal and environmental adaptability of the developed germplasm.

The development of new soybean cultivars most often requires thedevelopment of hybrid crosses (some exceptions being initial developmentof mutants directly through the use of the mutating agent, certainmaterials introgressed by markers, or transformants made directlythrough transformation methods) and the selection of progeny therefrom.Hybrids can be achieved by manual manipulation of the sexual organs ofthe soybean or by the use of male sterility systems. Breeders often tryto identify true hybrids by a readily identifiable trait or the visualdifferences between inbred and hybrid material. These heterozygoushybrids are then selected and repeatedly selfed and reselected to formnew homozygous soybean lines.

Mass and recurrent selection can be used to improve populations. Severalparents are intercrossed and plants are selected based on selectedcharacteristics like superiority or excellent progeny. Outcrossing to anumber of different parents creates fairly heterozygous breedingpopulations.

Pedigree breeding is commonly used with two parents that possessfavorable, complementary traits. The parents are crossed to form a F1hybrid. The progeny of the F1 hybrid is selected and the best individualF2s are selected; this selection process is repeated in the F3 and F4generations. The inbreeding is carried forward and at F5-F7 the bestlines are selected and tested in the development stage for potentialusefulness in a selected geographic area.

In backcross breeding a genetic allele or loci is transferred into adesirable homozygous recurrent parent. The trait is in the donor parentand is tracked into the recurrent parent. The resultant plant is likethe recurrent parent with the new desired allele or loci.

The single-seed descent method involves use of a segregating plantpopulation for harvest of one seed per plant. Each seed sample isplanted and the next generation is formed. When the F2 lines areadvanced to F6 each plant will be derived from a different F2. Thepopulation will decline due to failure of some seeds, so not all F2plants will be represented in the progeny.

New varieties must be tested thoroughly to compare their developmentwith commercially available soybeans. This testing usually requires atleast two years and up to six years of comparisons with other commercialsoybeans. Varieties that lack the entire desirable package of traits canbe used as parents in new populations for further selection or aresimply discarded. The breeding and associated testing process is 8 to 12years' of work prior to development of a new variety. Thousands ofvarietal lines are produced but only a few lines are selected in eachstep of the process. Thus the breeding system is like a funnel withnumerous lines and selections in the first few years and fewer and fewerlines in the middle years until one line is selected for the finaldevelopment testing.

The selected line or variety will be evaluated for its growth,development and yield. These traits of a soybean are a result of thevariety's genetic potential interacting with its environment. Allvarieties have a maximum yield potential that is predetermined by itsgenetics. This hypothetical potential for yield is only obtained whenthe environmental conditions are perfect. Since perfect growthconditions do not exist, field experimentation is necessary to providethe environmental influence and to measure its effect on the developmentand yield of the soybean. The breeder attempts to select for goodsoybean yield potential under a number of different environmentalconditions.

Selecting for good soybean yield potential in different environmentalconditions is a process that requires planning based on the analysis ofdata in a number of seasons. Identification of the varieties carrying asuperior combination of traits, which will give consistent yieldpotential, is a complex science. The desirable genotypic traits in thevariety can often be masked by other plant traits, unusual weatherpatterns, diseases, and insect damage. One widely employed method ofidentifying a superior plant with such genotypic traits is to observeits performance relative to commercial and experimental plants inreplicated studies. These types of studies give more certainty to thegenetic potential and usefulness of the plant across a number ofenvironments.

In summary, the goal of the soybean plant breeder is to produce new andunique soybeans and progeny of the soybeans for farmers' commercial cropproduction. To accomplish this the plant breeder painstakingly crossestwo or more varieties or germplasm. Then the results of this cross arerepeatedly selfed or backcrossed to produce new genetic patterns. Neweravenues for producing new and unique genetic alleles in soybeans includeintroducing mutations or transgenes into the genetic material of thesoybean are now in practice in the breeding industry. These geneticalleles can alter pest resistance such as insect resistance, nematoderesistance, herbicide resistance, or they can alter the plant's diseasetolerance, or its fatty acid compositions, the amount of oil produced,and/or the amino acid compositions of the soybean plant or its seed.

The traits a breeder selects for when developing new soybeans are drivenby the ultimate goal of the end user of the product. Thus if the goal ofthe end user is to resist a certain plant disease so overall more yieldis achieved, then the breeder drives the introduction of genetic allelesand their selection based on disease resistant levels shown by theplant. On the other hand, if the goal is to produce a specific oil, witha high level of oleic acid and a lower level of linoleic acid, then thebreeder may drive the selection of genetic alleles based on levels offatty acids in the seed and accept some lesser yield potentials or otherless desirable agronomic traits.

The new genetic alleles being introduced in to soybeans are widening thepotential uses and markets for the various products and by-products ofthe oil from the seed plants such as soybean. A major product extractedfrom soybeans is the oil in the seed. Soybean oil is employed in anumber of retail products such as cooking oil, baked goods, margarinesand the like. Another useful product is soybean meal, which is acomponent of many foods and animal feedstuffs.

SUMMARY OF THE INVENTION

One embodiment of the invention relates to seed of a soybean cultivardesignated S06-02KG294679. The invention relates to the plant from theseed designated S06-02KG294679, or the plant parts. The invention alsoencompasses a tissue culture of regenerable cells, cells or protoplastsbeing from a tissue selected from the leaf, pollen, stomatal cell,embryo, meristematic cell, root, root tip, anther, flower, ovule, seed,stem, pod, petal and the cells thereof.

The invention in one aspect covers a soybean plant, or parts thereof,having all of the physiological and morphological characteristics of thesoybean plant.

Another aspect of this invention is the soybean plant seed or derivedprogeny which contains a transgene which provides herbicide resistance,insect resistance, resistance to disease, resistance to nematodes, malesterility, or which alters the oil profiles, the fatty acid profiles,the amino acids profiles or other nutritional qualities of the seed.

The present invention further covers a method for producing a soybeanseed with the steps of crossing at least two parent soybean plants andharvesting the hybrid soybean seed, wherein at least one parent soybeanplant is the present invention. Another aspect of the invention coversthe hybrid soybean seed and the progeny soybean plant and resultantseed, or parts thereof from the hybrid seed or plant or its progeny.

In an additional aspect, the invention covers a method for producing asoybean progeny from the invention by crossing soybean lineS06-02KG294679 with a second soybean plant to yield progeny soybean seedand then growing progeny soybean seed.

Yet another aspect of the invention covers a method for a breedingprogram using plant breeding techniques which employ the soybean plantS06-02KG294679 as plant breeding material and performing breeding byselection techniques, backcrossing, pedigree breeding, marker enhancedselection, mutation and transformation.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the Geographic Segment Chart—GSEGC shows the breakout forgrain yield at standard moisture for S06-02KG294679 across geographiclocations.

FIG. 2 shows the Group Mean chart (GRP_MN=Group Mean) of Grain Yield atstandard moisture for S06-02KG294679. This chart shows YieldStability−Win>5% of trial mean, Tie + or −5% of trial mean, Loses<5% oftrial mean. The chart's vertical axis=yield of target variety, itshorizontal axis=location average yield. When the target variety line isabove the location average line this is desirable. The RSQ of the targetvariety shows a number. This number when it is closest to 1=yieldstability.

DETAILED DESCRIPTION

The following data is used to describe and enable the present soybeaninvention.

Trait Code Performance Trait Description VHNO Variety/Hybrid NumberYGSMN Grain Yield at Std MST (standard moisture) - YGSMN MRTYN MaturityDays from planting MRTYN HLDGR Harvest Lodging HLDGR PLHTN Plant Height(cm) PRR_R Phytophthora Root Rot Tol PRR_R IC_R Iron Chlorosis IC_RSDS_R Sudden Death Syndrome SDS_R SCL_R Sclerotinia White Mold SCL_RFELSR Frogeye Leaf Spot FELSR STR_R Shattering STR_R GLDGR Green LodgingGLDGR PLBRR Plant Branching PLBRR EMRGR Emergence EMRGR

Syngenta Soybean Data Collection Traits and Timing Trait Order GroupCode Description Timing Comments # Loc Stage 4 # Loc Stage 5 # Loc Stage6 Scale 1 HS RRG_R Presence of RR gene V2-V4, 4-7 DAYS Home Loc's HomeLoc's Home Loc's Roundup 1 = R, 5 ′2 seg, 9 = susc, AFTER SPRAYING 1 HSRUR_R Roundup Tolerance Yellowing R1-R2 Home Loc's Home Loc's Home Loc's1-9 scale, 1 = no yellowing, 9 is extreme yellowing 1 OTH EMRGREmergence - stand VE-V3 NK EPA_R = V2-V6, 2 4 1 to 9 (1 = best)establishment, uniformity and 2005 on use EMRGR vigor 2 DIS IC__R IronDeficiency Chlorsis August Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to9 (1 = best) Nursery 2 DIS ICFLR Iron Deficiency Chlorsis Initial flashV2 Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to 9 (1 = best) YellowFlash June-July Nursery 2 DIS ICR_R Iron Deficiency Chlorosis 2-3 weeksafter Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to 9 (1 = best)Recovery, (Stunting and/or ICFLR Nursery Green-up) 2 DIS PRR_RPhytophthora Root Rot Scheduled Internal Field St. Joe St. Joe 1 to 9 (1= best) for field Tolerance in pond Nursery tolerance 2 NEM SCN_R SCNField Rating June Internal Field Conesville, St. Conesville, St. JoeField = 1-9 (1 best) Nursery Joe 3 AGR FL_CR Flower Color R1 ConfirmedNumeric for EDC Home Loc's Home Loc's Home Loc's W = White; P = Purple;Seg = Mix 3 AGR PLCNR Plant canopy August (~R5-R6) Growth expressiveOpportunistic Opportunistic 1 to 9, 1 = Slender, locations. 5 =Intermediate, 9 = Bush 3 DIS ARSTR Asian Rust Rating When differencesOpportunistic Opportunistic Opportunistic 1 to 9 (1 = best) occur 3 DISBSR_R Brown Stem Rot When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 DIS CR__R Charcoal Rot Whendifferences Opportunistic Opportunistic Opportunistic 1 to 9 (1 = best)occur 3 DIS DPC_R Stem Canker (Northern) When differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 = best) occur 3 DIS DPM_R StemCanker (Southern) When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 DIS FELSR Frogeye Leaf Spot Whendifferences Opportunistic Opportunistic Opportunistic 1 to 9 (1 = best)occur 3 DIS SCL_R Sclerotinia White Mold When differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 = best) occur 3 DIS SDS_R SuddenDeath Syndrome When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 PERF GLDGR Green lodging R5 toR6 Where differences Opportunistic Opportunistic Opportunistic 1 to 9 (1best) 1 = All erect; 5 = occur 45 degrees; 9 = flat 4 AGR MRTYD MaturityDate (MMDD)—95% of R8 Home locations + 1- ~1-2 ~5 ~10 MMDD (Sept 10 =0910) plants in row shed leaves & 2 off site/station pods turned maturecolor 4 AGR HC.CR Hilum Color R8 Numeric EDC Home Loc's Home Loc's HomeLoc's G = Gray; BR = Brown; IB = Imperfect Black; Bl = Black; Y =Yellow; BF = Buff; IY = Imperfect Yellow; Seg = Mix 4 AGR PB_CRPubscence Color R8 - Harvest Numeric for EDC Home Loc's Home Loc's HomeLoc's G = Gray; T = Tawny; LT = Lt. Tawny; Seg = Mix 4 AGR PD_CR PodColor R8 - Harvest Numeric for EDC Home Loc's Home Loc's Home Loc's T =Tan; B = Brown; Seg = Mix 4 AGR PLBRR Plant branching R8 - HarvestGrowth expressive Optional Home Loc's Home Loc's 1 to 9, 1 = nobranching; 5 = locations. average branching; 9 = profuse branching 4 AGRPLHTN Plant Height (cm) Harvest Growth expressive Optional Home Loc'sHome Loc's Taken in cm locations. 4 DIS GS__R Green Stem Harvest Wheredifferences Opportunistic Opportunistic Opportunistic 1 to 9 (1 = best)occur 4 PERF HLDGR Harvest Lodging R8 - Harvest Where differencesOpportunistic Opportunistic Opportunistic 1 to 9 (1 best) 1 = All erect;5 = occur 45 degrees; 9 = flat 4 PERF HVAPR Harvest Appearance HarvestWhere differences Opportunistic Opportunistic Opportunistic 1 to 9, 1 =Excellent, 5 = Avg, 9 = occur Poor 4 PERF STR_R Shattering Harvest Hillplot planter, Opportunistic + Opportunistic + 1 to 9 (1 = best) 2006?Bay and St. Joe Bay and St. Joe Order 1 = Seedling, 2 = Vegetative, 3 =Reproductive to grain-fill, 4 = Maturity Opportunistic ratings shouldonly be collected if differences occur. At least one variety has arating of 5 or greater and there is a spread of 3 in the ratings.Roundup is a trademark of Monsanto Trait Definitions OpportunisticRatings developed in YT. Opportunistic ratings should only be collectedif differences occur. At least one variety has a rating of 5 or greaterand there is a spread of 3 in the ratings. (Scale 1-9, 1 = Best).Emergence (EMRGR) A rating of the uniform establishment and growth ofseedlings. Taken from V1-V3, (Scale 1-9). Maturity (MRTYD) The month andday (MMDD) when 95% of the main stem pods in the plot have reached theirmature color. Plant Height (PLHTN) The average measured plant height incm. Branching (PLBRR) Rating of the number of branches and theirrelative importance to yield. Taken at growth expressive locations.(Scale: 1-9, 1 = stick, no branching, 3 = 1 seed bearing branch, 5 =average branching or 2-3 seed bearing branches, 7 = 3-4 seed bearingbranches, 9 = profuse branching). Green Lodging (GLDGR) Rating based onthe average of plants leaning from vertical in R5 to R6 stage (Scale1-9, 1 to 9 (1 best) 1 = All erect; 5 = 45 degrees; 9 = flat). HarvestLodging (HLDGR) Rating based on the average of plants leaning fromvertical at harvest (scale 1-9, 1 to 9 (1 best) 1 = All erect; 5 = 45degrees; 9 = flat). Shatter (STR_R) Rating of pre-harvest loses based onamount of plants with open pods (Scale 1-9). Iron Deficiency Chlorosis(IC__R) final rating = average of initial Yellow Flash (ICFLR) andrecovery (ICR_R) taken 2-3 weeks after initial yellow flash. (Scale 1-9,1 = Best). LS means analysis unequal entries and reps between years.Soybean Cyst Nematode (CN_1R, CN_3R, CN_5R, CN14R) Greenhouse screen-30day screen using infested soil. Rating Scale based upon femalereproduction index on a susceptible check set where <10% = R; <30% = MR;<60% = MS; >60% = S. In priority order, the races screened include: 3,14, 1 & 5. Phytophthora Root Rot Field tolerance (PRR_R) or actual gene(RPS_T). Sudden Death Syndrome (SDS_R) based on leaf area affected,scale 1-9. Can be GH or field. Brown Stem Rot (BSR_R) Greenhousepot-root dip or field rating of leaf symptoms. (Scale 1-9) Root KnotNematode Arenaria (MA__R). Incognita (MI__R). Javanica (MJ__R) RKN).Scale 1-9. Stem Canker North (DPM_R) Southern (DPM_R). Scale 1-9.Sulfentrazone (SUL_R) Greenhouse nursery rating damage of multiplerates. Scale 1-9. Metributzin (MET_R) Greenhouse nursery rating damageof multiple rates. Scale 1-9. Hypocotyl Elongation (HYP_R) A rating of avariety's hypocotyl extension after germination when planted at a 5″depth in sand and maintained a warm germination environment for 10 days.(Scale 1 = Long, 5 = Intermediate, 9 = Short)

TRAIT DEFINITIONS

Hypocotyl Elongation (HYPO) A rating of a variety's hypocotyl extensionafter germination when planted at a 5″ depth in sand and maintained awarm germination environment for 10 days.

Seedling Establishment (EMG) A rating of the uniform establishment andgrowth of seedlings.

Peroxidase Activity (Perox)—seed protein peroxidase activity is definedas a chemical taxonomic technique to separate cultivars based on thepresence or absence of the peroxidase enzyme in the seed coat. Ratingsare POS=positive for peroxidase enzyme or NEG=negative for peroxidaseenzyme.

Plant Height (PLTHT) The average measured plant height in centimeters.

Branching (BRANCH) Rating of the number of branches and their relativeimportance to yield. This rating is taken at growth expressivelocations.

Green Lodging (GLODGE) Rating based on the average of plants leaningfrom vertical in R5 to R6 stage prior to maturity.

Harvest Lodging (LODGE) Rating based on the average of plants leaningfrom vertical at harvest. Lodging score (1=completely upright,9=completely prostrate),

Phytophthora Root Rot (PGR) or (PFT) Greenhouse pot—root dip method forPFT and hypodermic needle method for rating PGR.

Root Knot Nematode (RKN) Greenhouse screen—45 day screen root inoculatedwith eggs and juveniles. Rating Scale based upon female reproductionindex on a susceptible check set determined by number of galls present.

Stem Canker (STC) Based on number of lesions, scale 1-5.

Sulfentrazone (SULF) Authority™ (commercial herbicide) Greenhousenursery rating damage of multiple rates.

Metributzin (MET) Greenhouse nursery rating damage of multiple rates.

Brown Stem Rot (BSR or BSR_R) This disease is caused by the fungusPhialophora gregata. The disease is a late-season, cool-temperature,soilborne fungus which in appropriate favorable weather can cause up to30 percent yield losses in soybean fields. BSR information is gatheredin a greenhouse with a plant in a pot then a root dip procedure isemployed. BSR_R is an opportunistic field rating scale is 1-9.

Sudden Death Syndrome (SDS or SDS_R) This disease is caused byslow-growing strains of Fursarium solani that produce bluish pigments inculture. The disease is a mid to late season, soil borne disease thatoccurs in soybean fields with high yield potential. Yield losses may betotal or severe in infected fields. Sudden Death Syndrome (SDS_R) isbased on leaf area affected. The scale used for these tests is 1-5 or ifidentified as SDS_R the scale is 1-9.

Sclerotinia White Mold (SCL_R) This disease is caused by the fungalpathogen Sclerotinia sclerotium. The fungus can overwinter in the soilfor many years as sclerotia and infecting plants in prolonged periods ofhigh humidity or rainfall. Yield losses may be total or severe ininfected fields. Sclerotinia White Mold (SCL_R) rating is a field rating(1-9 scale) based the percentage of wilting of dead plants in a plot.

Frog Eye Leaf Spot (FELSR) This is caused by the fungal pathogenCercospora sojina. The fungus survives as mycelium in infected seeds andin infested debris. With adequate moisture new leaves become infected asthey develop until all the leaves are infected. Yield losses may be upto 15% in severe infected fields. Frog Eye Leaf Spot (FELSR) rating is afield rating (1-9 scale) based the percentage of leaf area affected.

Soybean Cyst Nematode (SCN) The Soybean Cyst Nematode Heteroderaglycines, is a small plant-parasitic roundworm that attacks the roots ofsoybeans. Soybean Cyst Nematode (SCN) for purposes of these tests isdone as a greenhouse screen—30 day screen using infested soil. Therating scale is based upon female reproduction index on a susceptiblecheck set where <10%=R (RESISTANT); <30%=MR (MODERATELY RESISTANT);<60%=MS (MODERATELY SUSPECTIBLE); >60%=S (SUSPECTIBLE). In priorityorder, the screening races include: 3, 14, & 1.

Maturity Date (MAT or MRTYN) Plants are considered mature when 95% ofthe pods have reached their mature color. For MAT, the number of days iscalculated from August 31 or MRTYN is calculated from the number of daysfrom planting date.

Relative Maturity Group (RM) Industry Standard for varieties groups,based day length or latitude. Long day length (northern areas in theNorthern Hemisphere) is classified as (Groups 000,00,0,). Mid daylengths variety groups lie in the middle (Groups I-VI). Very short daylengths variety groups (southern areas in Northern Hemisphere) areclassified as (Groups VII, VIII, IX).

Seed Yield (YSGMN) The actual grain yield at harvest reported in theunits bushels/acre.

Shattering (SHAT or STR_R) The rate of pod dehiscence prior to harvest.Pod dehiscence is the process of beans dropping out of the pods. Shatter(SHAT) for these tests the rating of pre-harvest loses is based onamount of plants with open pods on a scale of 1-5, STR_R on a scale 1-9.

Plant Means the plant, the plant's cells, plant protoplasts, plant cellsof tissue culture from which soybean plants can be regenerated, plantcalli, plant clumps, and plant cells that are intact in plants or partsof the plants, such as pollen, nodes, roots, flowers, seeds, pods,leaves, stems, pod and the like.

Definitions of Staging of Development

The plant development staging system employed in the testing of thisinvention divides stages as vegetative (V) and reproductive (R). Thissystem accurately identifies the stages of any soybean plant. However,all plants in a given field will not be in the stage at the same time.Therefore, each specific V or R stage is defined as existing when 50% ormore of the plants in the field are in or beyond that stage.

The first two stages of V are designated a VE (emergence) and VC(cotyledon stage). Subdivisions of the V stages are then designatednumerically as V1, V2, V3 through V (n). The last V stage is designatedas V (n), where (n) represents the number for the last node stage of thespecific variety. The (n) will vary with variety and environment. Theeight subdivisions of the reproductive stages (R) states are alsodesignated numerically. R1=beginning bloom; R2=full bloom; R3=beginningpod; R4=full pod; R5=beginning seed; R6=full seed; R7=beginningmaturity; R8=full maturity.

Soybean Cultivar

The present invention is S06-02KG294679. This soybean is developed foruse of the beans. To be sold commercially in the Midsouth and Southeastwhere other Maturity Group V soybeans are grown. Specific area wherebest adaptation occurs includes: areas that require resistance tosoybean cyst nematode (Heterodera glycines) race 3, southern root knotnematode (Meloidogyne incognita) and frogeye leaf spot (Cercosporasojina). The present invention is also adapted to high-yield, lodgingprone environments.

This soybean variety in one embodiment carries one or more transgenes,for example, the glyphosate tolerance transgene, a desaturase gene orother transgenes. Another embodiment of the invention does not carry anyherbicide resistance traits. In yet another embodiment of the invention,the soybean does not carry any transgenes but carries alleles for aphidresistance, cyst nematode resistance and/or brown stem rot or the like.

The traits of the invention are listed below.

TRAITS Plant Characteristics RR ® Y STS ® N Flower Color P PubescenceColor T Pod Color T Hilum Color BL % Protein 13% mst. 38.7 % Oil @ 13%mst. 19.2 Seed Size/Lb Stem Termination 1.0 Plant Health Rps GenePhytophthora Root Rot Tolerance 3.8 SCN RACE 1 FI % 17.0 SCN RACE 3 FI %1.0 SCN RACE 5 FI % SCN RACE 14 FI % Root Knot Nematode - Incognita 1.0Root Knot Nematode - Arenaria Stem Canker (Southern) 8.0 Stem CankerTolerance (Southern) 2.0 Rps gene indicates the specific gene forresistance but if none are indicated then none are known to be present Y= Yes, has trait. N = no does not contain trait RoundUp Ready ®,Roundup ® and Roundup ® Ultra are trademarks of Monsanto Company. STS ®is a trademark of DuPont. Ratings are on a 1 to 9 scale with 1 being thebest.

This plant does show a good general field resistance to the disease.

The present invention provides methods and composition relating toplants, seeds and derivatives of the soybean cultivar S06-02KG294679.Soybean cultivar S06-02KG294679 has superior characteristics. TheS06-02KG294679 line has been selfed sufficient number of generations toprovide a stable and uniform plant variety.

Cultivar S06-02KG294679 shows no variants other than expected due toenvironment or that normally would occur for almost any characteristicduring the course of repeated sexual reproduction. Some of the criteriaoften used to select in various generations include: seed yield,emergence, appearance, disease tolerance, maturity, plant height, andshattering data.

The inventor believes that 506-02KG294679 is similar to the comparisonvarieties shown in the last table. However, as shown in the table,S06-02KG294679 differs from these cultivars.

Direct comparisons were made between S06-02KG294679 and the listedcommercial varieties. Traits measured included yield, maturity,moisture, lodging, plant height, branching, field emergence, andshatter. The results of the comparison are presented in below. Thenumber of tests in which the varieties were compared is shown.

The present invention S06-02KG294679 can carry genetic engineeredrecombinant genetic material to give improved traits or qualities to thesoybean. For example, but not limited to the present invention can carrythe glyphosate resistance gene for herbicide resistance as taught in theMonsanto patents (WO92/00377, WO92/04449, U.S. Pat. No. 5,188,642 andU.S. Pat. No. 5,312,910) or STS mutation for herbicide resistance.Additional traits carried in transgenes or mutation can be transferredinto the present invention. Some of these genes include genes that givedisease resistance to sclerotinia such as the oxalate oxidase (Ox Ox)gene as taught in PCT/FR92/00195 Rhone Polunc and/or an OxalateDecarboxylase Decarboxylate gene for disease resistance or genesdesigned to alter the soybean oil within the seed such as desaturase,thioesterase genes (shown in EP0472722, U.S. Pat. No. 5,344,771) orgenes designed to alter the soybean's amino acid characteristics. Thisline can be crossed with another soybean line that carries a gene thatacts to provide herbicide resistance or alter the saturated and/orunsaturated fatty acid content of the oil within the seed, or the aminoacid profile of the seed.

The present invention S06-02KG294679 is employed in a number of plotrepetitions to establish trait characteristics in differentenvironments. The present invention is grown in a number of regions fortesting and researching the invention in various environmentalinteractions.

Geographic Summary

The target variety yield is given as a percent of the trial average atall locations shown in FIG. 1 and each geographic segment West to Eastor Central (Cntrl) to South East where there are three or morelocations. The plots for these trials are two row 17.5 foot long plotsplanted in 30-inch spaces. The plants in the plots are a combination ofexperimental material and commercial material. There are usually 36varieties and there are approximately 300 plants of each variety withtwo replications in about 20-25 locations. The data in FIG. 1 is onlycharted if there are at least 3 or more locations. N>=3

The present invention differs from the mean of the comparison commercialand experimental soybean lines in that it substantially exceeding acrossall regions the mean of the group of soybeans (GRP_MN) that were testedand displayed in this geographic segment chart (GSEGC). When all of theregions that the testing data for the present invention are included thepresent invention is about 4.1% above the group mean. However, in themiddle South region this invention provides even better levels of yieldwhen compared with the mean of that group. In the Mid South the presentinvention is exceeding the group mean by 6.1%. The data in the Deltaregion is also shows that the present invention is overachievingcompared to the Group Mean. Some of the yield may be due to the lack ofroot damage due to the resistance to both forms of nematode.

The present invention S06-02KG294679 (identified herein as 02KG294679)is employed in a trial for testing a number of environments. The resultsof the grain yield at standard moisture are shown in the chart as shownin FIG. 2. The present invention is a variety that will out yield mostsoybeans in a high yielding environment in a mid yielding environmentand in the lowest yielding environments. If the environment is extremelylow yielding the present invention tends to perform slightly better thanthe expectations in that environment but when the environment is highyielding the present invention shows a larger difference between itsyield and the expectations of the environment. These tests allow theusefulness of the invention to be shown in light of the differentenvironmental genetic interactions.

Each of these lines has their own positive traits. Each of these linesis different from the present invention. The present invention hashigher yield at standard moisture than does the comparison line. Thepresent invention shows an excellent rating for resistance to FrogeyeLeaf Spot and a good rating for PRR_R. The ID-R rating is slightlyhigher (1=best) than the compared line. Thus, indicating less IDCresistance than is shown by the other lines. The yield and other data isa snapshot of each of these lines' results in the specific environmentand will differ when other environmental interactions are measured.

This invention also is directed to methods for producing a new soybeanplant by crossing a first parent plant with a second parent plantwherein the first or second parent plant is the present invention.Additionally, the present invention may be used in the varietydevelopment process to derive progeny in a breeding population orcrossing. Further, both first and second parent plants can come from thesoybean line S06-02KG294679. A variety of breeding methods can beselected depending on the mode of reproduction, the trait, and thecondition of the germplasm. Thus, any such methods using theS06-02KG294679 are part of this invention: selfing, backcrosses,recurrent selection, mass selection and the like.

The scope of the present invention includes any use on S06-02KG294679 ofmarker methods. Through the use of markers such as SSRs, RFLP's, SNPs,Ests, AFLPs, gene primers, and the like to identify genetic alleleswhich can be identified and bred with marker assistance into the presentinvention with little to no superfluous germplasm being dragged into thepresent invention. This results in formation of the present inventionplus for example, cyst nematode resistance, brown stem rot resistance,aphid resistance, phytothora resistance, IDC resistance, BT genes ormale sterility genes or glyphosate tolerance genes or drought tolerancegenes or alleles and the like. Transgenes maybe directly introduced intothe cultivar using genetic engineering and transformation techniqueswell known in the art, some of which are described above, or areoriginally introduced into a donor parent using genetic engineering andtransformation techniques, and using the donor in a marker assistedtrait conversion process, the trait is moved for example bybackcrossing. A transgene typically comprises a nucleotide sequencewhose expression is responsible or contributes to the trait, under thecontrol of a promoter capable of directing the expression of thenucleotide sequence at the desired time in the desired tissue or part ofthe plant. Constitutive, tissue-specific or inducible promoters all havedifferent purposes and each could be employed. The transgene may alsocomprise other regulatory elements such as for example translationenhancers or termination signals. The transgene may be adapted to betranscribed and translated into a protein, or to encode RNA in a senseor antisense orientation such that it is not translated or onlypartially translated.

The scope of the present invention also includes any use onS06-02KG294679 of transformation methods. Transformation methods aremeans for integrating new genetic coding sequences (transgenes) into theplant's genome by the incorporation of these sequences into a plantthrough man's assistance. Many dicots including soybeans can easily betransformed with Agrobacterium. Methods of introducing desiredrecombinant DNA molecules into plant tissue include the direct infectionor co-cultivation of plant cells with Agrobacterium tumefaciens, Horschet al., Science, 227:1229 (1985). Descriptions of Agrobacterium vectorsystems and methods are shown in Gruber, et al., “Vectors for PlantTransformation, in Methods in Plant Molecular Biology & Biotechnology”in Glich et al., (Eds. pp. 89-119, CRC Press, 1993). Transformed plantsobtained via protoplast transformation are also intended to be withinthe scope of this invention. The most common method of transformationafter the use of agrobacterium is referred to as gunning ormicroprojectile bombardment. This process has small gold-coatedparticles coated with DNA (including the transgene) shot into thetransformable material. Techniques for gunning DNA into cells, tissue,explants, meristems, callus, embryos, and the like are well known in theprior art. The DNA used for transformation of these plants clearly maybe circular, linear, and double or single stranded. Usually, the DNA isin the form of a plasmid. The plasmid usually contains regulatory and/ortargeting sequences which assists the expression of the gene in theplant. The methods of forming plasmids for transformation are known inthe art. Plasmid components can include such items as: leader sequences,transit polypeptides, promoters, terminators, genes, introns, markergenes, etc. The structures of the gene orientations can be sense,antisense, partial antisense, or partial sense: multiple gene copies canbe used.

After the transformation of the plant material is complete, the nextstep is identifying the cells or material, which has been transformed.In some cases, a screenable marker is employed such as thebeta-glucuronidase gene of the uidA locus of E. coli. Then, thetransformed cells expressing the colored protein are selected for eitherregeneration or further use. In many cases, a selectable markeridentifies the transformed material. The putatively transformed materialis exposed to a toxic agent at varying concentrations. The cells nottransformed with the selectable marker, which provides resistance tothis toxic agent, die. Cells or tissues containing the resistantselectable marker generally proliferate. It has been noted that althoughselectable markers protect the cells from some of the toxic affects ofthe herbicide or antibiotic, the cells may still be slightly affected bythe toxic agent by having slower growth rates. If the transformedmaterials are cell lines then these lines are used to regenerate plants.The cells' lines are treated to induce tissue differentiation. Methodsof regeneration of plant from cellular material are well known in theart. The plants from the transformation process or the plants resultingfrom a cross using a transformed line or the progeny of such plants aretransgenic plants that carry the transgene.

DEPOSIT INFORMATION

Applicants have made a deposit of at least 2500 seeds of soybeancultivar S06-02KG294679 with the American Type Culture Collection(ATCC), Manassas, Va. 20110 on Apr. 8, 2008 and designated PTA-9144. Theseeds were tested on and found to be viable. Access to this deposit willbe available during the pendency of the application to the Commissionerfor Patents and persons determined by the Commissioner to be entitledthereto upon request. Upon granting of a patent on any claims in theapplication, the Applicants will make the deposit available to thepublic pursuant to 37 CFR §1.808. Additionally, Applicants will meet therequirements of 37 CFR §1.801-1.809, including providing an indicationof the viability of the sample when the deposit is made. The ATCCdeposit will be maintained in that depository, which is a publicdepository, for a period of 30 years, or 5 years after the last request,or for the enforceable life of the patent, whichever is longer, and willbe replaced if it becomes nonviable during that period.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though, that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

1. A soybean seed designated S06-02KG294679, a sample of said seeddeposited under ATCC Accession No. PTA-9144.
 2. A plant, or partsthereof, produced by growing the seed of claim
 1. 3. Pollen of the plantof claim
 2. 4. A soybean plant, or parts thereof, having all of thephysiological and morphological characteristics of the soybean plant ofclaim
 2. 5. A tissue culture of regenerable cells of the soybean plantof claim
 2. 6. The tissue culture according to claim 5, wherein thecells are obtained from the group consisting of leaf, pollen, embryo,meristematic cell, root, root tip, anther, stomatal cell, flower, seed,stem and pod.
 7. A soybean plant regenerated from the tissue culture ofclaim 6, having all of the morphological and physiologicalcharacteristics of soybean cultivar S06-02KG294679.
 8. A method forproducing a soybean seed comprising crossing two soybean plants andharvesting the resultant soybean seed, wherein at least one soybeanplant is the soybean plant of claim
 2. 9. A method for producing ahybrid soybean seed comprising crossing the soybean plant according toclaim 2 with a second soybean plant and harvesting the resultant hybridsoybean seed.
 10. A method for producing a S06-02KG294679-derivedsoybean plant, comprising: a) crossing soybean line S06-02KG294679, asample of said line deposited under ATCC Accession No. PTA-9144, with asecond soybean plant to yield progeny soybean seed; and b) growing saidprogeny soybean seed to yield said S06-02KG294679-derived soybean plant.11. The method of claim 8, wherein the second soybean plant istransgenic.
 12. The method of claim 11 wherein the transgenic soybeanplant contains genetic material conferring a trait selected from thegroup consisting of herbicide resistance, nematode resistance, insectresistance, resistance to disease, and male sterility.
 13. The method ofclaim 12 wherein the resistance to disease is through an oxalate oxidaseencoding polynucleotide sequence or an oxalate decarboxylase encodingpolynucleotide sequence.